Aerospray GramWescor - Elitech Group
PRET LA CERERE
Aerospray provides many advantages over hand staining or automated dip type stainers, including speed, economy, consistent performance, and no cross-contamination.
The Aerospray stainer stains microscope slides smeared with materials containing microorganisms such as culture materials, body fluids, or exudates. Elitech gram reagents are tailored to give superb results in the Aerospray stainer.
The Aerospray stainer stains microscope slides smeared with materials containing micro-organisms such as culture materials, body fluids, or exudates. ELITech gram reagents are tailored to give superb results in the Aerospray stainer. and stains them dark blue-to- black. The excess stain is then spun off and washed away with deionized water to prevent precipitate formation outside stained cells. stainer provides a choice of decolorizer cycles ranging from 1 to 9, allowing you to compensate for thick specimens, or other pos- sible variables. With the ELITech system, you can reproducibly differentiate sensitive organisms in normal smears. With its rapid staining cycle and high throughput capability, the Aerospray stainer is a convenient, cost effective way to increase gram staining capacity.
Iodine reagent is then sprayed onto the slide. The iodine penetrates the cells and forms a precipitate (mordant) with the crystal violet dye. Excess iodine is spun off and rinsed from the slide with deionized water.
Reagents are applied from atomizing spray nozzles onto microscope slides containing the specimens. Staining is rapid and precise. Specimens contact only fresh stain, avoiding specimen cross-contamination or contact with contaminated reagents.
DRYING DECOLORIZING AND COUNTERSTAIN
Decolorizer is then applied to remove the crystal violet-iodine complex until only the gram-negative organisms are decolorized. Safranin or fuchsin (contained in the decolorizer) simultaneously counter-stains the gram-negative cells pink-to-red.
Decolorization is the critical step in proper gram differentiation and unless properly controlled can lead to errors. The Aerospray The slides are spun at about 950 RPM for 45 seconds. This draws air through the stainer and dries the slides. Slides emerge from the stainer dry and ready for the microscope. With its rapid staining cycle and high throughput capability, the Aerospray stainer is a convenient, cost effective way to stain gram specimens.
Specimens can be heat-fixed and stained directly, or the automatic fixation feature can be used to provide convenient and reproducible specimen fixation.
Once slides are fixed, they are sprayed with crystal violet, a positively charged (basic) dye which rapidly penetrates microbial cells